منابع مشابه
Investigating translational repression by microRNAs in Arabidopsis.
Regulation of gene expression by microRNAs (miRNAs) is an important facet of many developmental and physiological processes in both plants and animals. miRNAs are small (;20 to 24 nucleotides) RNAs that recognize target genes based on sequence complementarity and typically act to repress expression of the target gene, either through cleavage of the target mRNA or translational repression indepe...
متن کاملMicroarray-based analysis of stress-regulated microRNAs in Arabidopsis thaliana.
High-salinity, drought, and low temperature are three common environmental stress factors that seriously influence plant growth and development worldwide. Recently, microRNAs (miRNAs) have emerged as a class of gene expression regulators that have also been linked to stress responses. However, the relationship between miRNA expression and stress responses is just beginning to be explored. Here,...
متن کاملHighly specific gene silencing by artificial microRNAs in Arabidopsis.
Plant microRNAs (miRNAs) affect only a small number of targets with high sequence complementarity, while animal miRNAs usually have hundreds of targets with limited complementarity. We used artificial miRNAs (amiRNAs) to determine whether the narrow action spectrum of natural plant miRNAs reflects only intrinsic properties of the plant miRNA machinery or whether it is also due to past selection...
متن کاملNuclear processing and export of microRNAs in Arabidopsis.
In mammalian cells, the nuclear export receptor, Exportin 5 (Exp5), exports pre-microRNAs (pre-miRNAs) as well as tRNAs into the cytoplasm. In this study, we examined the function of HASTY (HST), the Arabidopsis ortholog of Exp5, in the biogenesis of miRNAs and tRNAs. In contrast to mammals, we found that miRNAs exist as single-stranded 20- to 21-nt molecules in the nucleus in Arabidopsis. This...
متن کاملBiochemical evidence for translational repression by Arabidopsis microRNAs.
MicroRNAs (miRNAs) regulate gene expression posttranscriptionally through RNA silencing, a mechanism conserved in eukaryotes. Prevailing models entail most animal miRNAs affecting gene expression by blocking mRNA translation and most plant miRNAs, triggering mRNA cleavage. Here, using polysome fractionation in Arabidopsis thaliana, we found that a portion of mature miRNAs and ARGONAUTE1 (AGO1) ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant Physiology
سال: 2008
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.104.900244